Description
Certified Particle Size Polystyrene – Nanosphere Size Standards
These Nanosphere Size Standards are highly uniform polystyrene spheres calibrated in billionths of a meter (nanometers) with NIST traceable methodology. One nanometer is 0.001 micrometer ( m) or 10 Angstrom units.
Nanosphere Size Standards are packaged as aqueous suspensions in 15 milliliter (ml) dropper-tipped bottles. The concentrations are optimized for ease of dispersion and colloidal stability. The spheres have a density of 1.05 g/cm3 and an index of refraction of 1.59 @ 589nm (23 C).
Methods used to calibrate the diameter of the Nanospheres include adaptions of photon correlation spectroscopy (PCS) and transmission electron microscopy (TEM). PCS uses scattered laser light to correlate the diameter of suspended particles with their Brownian motion. PCS is also referred to as quasi-elastic light scattering or dynamic light scattering.
Nanopshere Size Standards are ideal for the calibration of electron and atomic force microscopes. They are also used in laser light-scattering studies and colloidal systems research. The 20 to 900nm range of diameters is convenient for checking the sizes of bacteria, viruses, ribosomes and sub-cellular components
| Sphere Size and Size Deviation Table | ||||
| Prod. No. | Nom Diameter | Certified Mean Diameter | Size Distribution Std Dev. & C.V. |
Solids Content |
|---|---|---|---|---|
| 610-50 | 20nm | 21nm 1.5nm | not determined | 1% |
| 610-53 | 50nm | 46nm 2.0nm | 7.2nm (15.7%) | 1% |
| 610-56 | 80nm | 81nm 2.7nm | 5.8nm (7.2%) | 1% |
| 610-58 | 100nm | 97nm 3nm | 4.5nm (4.6%) | 1% |
| 610-60 | 150nm | 151nm 4nm | 3.2nm (2.1%) | 1% |
| 610-61 | 200nm | 200nm 6nm | 3.4nm (1.7%) | 1% |
| 610-63 | 240nm | 240nm 6nm | 3.7nm (1.5%) | 1% |
| 610-66 | 350nm | 350nm 7nm | 4.7nm (1.3%) | 1% |
| 610-69 | 500nm | 491nm 4nm | 6.3nm (1.3%) | 1% |
| 610-73 | 600nm | 596nm 6nm | 7.7nm (1.3%) | 1% |
| 610-76 | 900nm | 903nm 9nm | 9.3nm (1.0%) | 1% |
