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AFM Gold Calibration Kit
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Characterized colloidal gold particles for:
- Characterization of scanning tip geometry
- Reliable calibration of the vertical scale of piezoelectric response
- Characterizing vertical dimensions of coadsorbed biomolecules
Three sizes of colloidal gold particles are available in a convenient kit form. The kit contains 8 numbered 15mm AFM discs with mica attached for calibration and tip characterization.
Remaining colloidal gold can be used for coadsorption with biomolecules or other samples.
16200 and 16205 Kits Contain:
- PELCO® 15mm AFM Disc Carrier
- 15mm AFM Discs, numbered with 9.9mm Mica Discs attached, 8 ea., in a 16214 PELCO® Disc Carrier
- PELCO® AFM Disc Pickup Tool
- Gold Colloid, 5nm Range, 500µl
- Gold Colloid, 15nm Range, 500µl
- Gold Colloid, 30nm Range, 500µl
- Poly-L-Lysine, 0.1%, 500µl
Fig. 1. AFM scan of 7.7, 14.6 and 28.4nm gold particles.
Note: Scan (arrow) of 28.4nm particle indicates the presence of a tip artifact.
ABSTRACT:
Colloidal gold particles have multiple uses as three-dimensional atomic force microscopy imaging standards because they are incompressible, monodisperse, and spherical. The spherical nature of the particles can be exploited to characterize scanning tip geometry.
As uniform spheres, colloidal gold particles may be used to calibrate the vertical dimensions of atomic force microscopy at the nanometer level. The monodisperse and incompressible nature of the gold can be used to characterize the vertical dimension of coadsorbed biomolecules.
Simultaneous measurements of gold with tobacco mosaic virus show that, at the same applied vertical force, the tobacco mosaic virus is undamaged by blunt tips but is compressed or disintegrated under sharper scanning styli, suggesting that specimen degradation is partly a pressure-dependent effect.
Vesenka J, Manne S, Giberson R, Marsh T, and Henderson E, 1993. Colloidal Gold Particles as an Incompressible Atomic Force Microscope Imaging Standard for Assessing the Compressibility of Biomolecules. Biophysical Journal 65:1-6.
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PELCO AFM Gold Standard Kit
Three sizes of colloidal gold particles are available in a convenient kit form.
no. 16200
Description
AFM Gold Calibration Kit
Colloidal gold particles have multiple uses as three-dimensional atomic force microscopy imaging standards because they are incompressible, monodisperse, and spherical.
The spherical nature of the particles can be exploited to characterize scanning tip geometry.
As uniform spheres, colloidal gold particles may be used to calibrate the vertical dimensions of atomic force microscopy at the nanometer level.
The monodisperse and incompressible nature of the gold can be used to characterize the vertical dimension of coadsorbed biomolecules.
Simultaneous measurements of gold with tobacco mosaic virus show that, at the same applied vertical force, the tobacco mosaic virus is undamaged by blunt tips but is compressed or disintegrated under sharper scanning styli, suggesting that specimen degradation is partly a pressure-dependent effect.
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Same as 16200 plus 2 additional gold sizes, 10 & 20nm range
Three sizes of colloidal gold particles are available in a convenient kit form.
no. 16205
Description
AFM Gold Calibration Kit
Colloidal gold particles have multiple uses as three-dimensional atomic force microscopy imaging standards because they are incompressible, monodisperse, and spherical.
The spherical nature of the particles can be exploited to characterize scanning tip geometry.
As uniform spheres, colloidal gold particles may be used to calibrate the vertical dimensions of atomic force microscopy at the nanometer level.
The monodisperse and incompressible nature of the gold can be used to characterize the vertical dimension of coadsorbed biomolecules.
Simultaneous measurements of gold with tobacco mosaic virus show that, at the same applied vertical force, the tobacco mosaic virus is undamaged by blunt tips but is compressed or disintegrated under sharper scanning styli, suggesting that specimen degradation is partly a pressure-dependent effect.
Technical Note
Technical Note
|
Download Technical Note |
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