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QUANTIFOIL® TEM Substrates
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Support Grids or Substrates for Transmission Electron Microscopy
Quantifoil® Holey Carbon Film is a perforated support foil with a p re-defined hole size, shape and arrangement.
They are offered with circular and square, orthogonally arranged holes. The film is mounted on a standard Ø3mm Cu, Au, or Ni TEM grid with 200, 300 or 400 mesh. They have advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.
The final thickness of the foil is about 20nm, although only 10nm of carbon is evaporated onto the plastic.
Four reasons to use Quantifoil®:
- With the known size of the pore of the Quantifoil®, one can estimate the size of a particle observed directly.
- With the defined size and pitch of the holes in the Quantifoil<sup® one can use automated electron microscopy for the first time.
- The Quantifoil® 7/2 gives a good portion of “free” area.
- Hole size and hole distribution uniform over a wide range.
Available geometries
| QUANTIFOIL Type | Hole Size in µm | Hole distance in µm | Pitch in µm |
|---|---|---|---|
| R 0.6/1 | 0.6 | 1.0 | 1.6 |
| R 1/2 | 1.0 | 2.0 | 3.0 |
| R 1/4 | 1.0 | 4.0 | 5.0 |
| R 1.2/1.3 | 1.2 | 1.3 | 2.5 |
| R 1.2/20 | 1.2 | 20.0 | 21.2 |
| R 2/1 | 2.0 | 1.0 | 3.0 |
| R 2/2 | 2.0 | 2.0 | 4.0 |
| R 2/4 | 2.0 | 4.0 | 6.0 |
| R 3/3 | 3.0 | 3.0 | 6.0 |
| R 3/5 | 3.0 | 5.0 | 8.0 |
| R 3.5/1 | 3.5 | 1.0 | 4.5 |
| R 5/10 | 5.0 | 10.0 | 15.0 |
| R 5/20 | 5.0 | 20.0 | 25.0 |
| R 6/6.5 | 6.0 | 6.5 | 12.5 |
| R 6/100 | 6.0 | 100.0 | 106.0 |
| R 7/2 | 7.0 | 2.0 | 9,0 |
| R 10/5 | 10.0 | 5.0 | 15.0 |
| R 10/10 | 10.0 | 10.0 | 20.0 |
| R 10/20 | 10.0 | 20.0 | 30.0 |
| R 17/5 | 17.5 | 5.0 | 22.5 |
| R 35/5 | 35.0 | 5.0 | 40,0 |
| R 35/10 | 35.0 | 10.0 | 45,0 |
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Product
Description
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Product
Quantifoil Array of 0,6 m Round Holes R0,6/1
Quantifoil, Array of 0,6 m Diameter Holes with 1 m Separation
no. S180-1 to S180-1
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 1 m Round Holes R1/2
Quantifoil, Array of 1 m Diameter Holes with 2 m Separation
no. S197-1 to S197-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 1 m Round Holes R1/4
Quantifoil, Array of 1 m Diameter Holes with 4 m Separation
no. S176-1 to S176-8
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 1.2 m Round Holes R1,2/1,3
Quantifoil, Array of 1,2 m Diameter Holes with 1,3 m Separation
no. S143-1 to S143-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 1,2 m Round Holes R1.2/20
Quantifoil, Array of 1,2 m Diameter Holes with 20 m Separation
no. S198-1
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 2 m Round Holes R2/1
Quantifoil, Array of 2 m Diameter Holes with 1 m Separation
no. S174-1 to S174-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil Array of 2 m Round Holes R2/2
Quantifoil, Array of 2 m Diameter Holes with 2 m Separation
no. S173-1
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 2 m Round Holes R2/4
Quantifoil, Array of 2 m Diameter Holes with 4 m Separation
no. S175-1 to S175-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 3 m Round Holes R3/3
Quantifoil, Array of 3 m Diameter Holes with 3 m Separation
no. S192-1 to S192-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 3 m Round Holes R3/5
Quantifoil, Array of 3 m Diameter Holes with 5 m Separation
no. S193-1 to S193-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil Array of 3,5 m Round Holes R3,5/1
Quantifoil, Array of 3,5 m Diameter Holes with 1 m Separation
no. S177-1 to S177-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil Array of 5 m Round Holes R5/10
Quantifoil, Array of 5 m Diameter Holes with 10 m Separation
no. S186-1 to S186-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil on an Array of 5 m Round Holes R5/20
Quantifoil, Array of 5 m Diameter Holes with 20 m Separation
no. S181-1 to S181-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil Array of 6 m Round Holes R6/6.5
Quantifoil, Array of 6 m Diameter Holes with 6,5 m Separation
no. S185-1 to S185- 9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil on an Array of 6 m Round Holes R6/100
Quantifoil, Array of 6 m Diameter Holes with 100 m Separation
no. S194-1 to S194-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil on an Array of 10 m Round Holes R10/5
Quantifoil, Array of 10 m Diameter Holes with 5 m Separation
no. S183-1 to S183-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil Array of 10 m Round Holes R10/10
Quantifoil, Array of 10 m Diameter Holes with 10 m Separation
no. S182-1 to S182-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil on an Array of 10 m Round Holes R10/20
Quantifoil, Array of 10 m Diameter Holes with 20 m Separation
no. S187-1 to S187-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
Order
Product
Description
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Product
Quantifoil Array of 17,5 m Round Holes R17/5
Quantifoil, Array of 17,5 m Diameter Holes with 5 m Separation
no. S184-1 to S184-9
Description
Quantifoil with round Openings
Quantifoil with round Openings are mainly used in cryo-electron microscopy.
The roundness of the holes brings advantages in terms of the Formation of a layer of ice with a constant thickness.
The required hole sizes depend on the magnification, and if you want to have a carrier film in the image or not.
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Product
Description
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Product
Quantifoil Array of 35 m Square Holes S35/5
Quantifoil, Array of 35 m Square Holes with 5 m Separation
no. S195-1 to S195-9
Description
Quantifoil with Square Openings
QUANTIFOIL with square holes and relatively narrow bars can be used in electron microscopy to carry a thin carbon film that on it self is too fragile.
Alternatively, this porous film can carry an object directly, which is larger than the holes.
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Description
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Quantifoil on an Array of 35 m Square Holes S35/10
Quantifoil, Array of 35 m Square Holes with 10 m Separation
no. S196-1 to S196-9
Description
Quantifoil with Square Openings
QUANTIFOIL with square holes and relatively narrow bars can be used in electron microscopy to carry a thin carbon film that on it self is too fragile.
Alternatively, this porous film can carry an object directly, which is larger than the holes.
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Product
Description
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Quantifoil Array of 7 m square Holes
Quantifoil, Array of 7 m Square Holes with 2 m Separation
no. S117-1 to S117-9
Description
Quantifoil with Square Openings
QUANTIFOIL with square holes and relatively narrow bars can be used in electron microscopy to carry a thin carbon film that on it self is too fragile.
Alternatively, this porous film can carry an object directly, which is larger than the holes.
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Description
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Quantifoil Substrate Hexagonal Holes
Hexagonal holes with a hole size of 26 m (assumed to be inside the appropriate circle), bar width of about 15 microns and a hole distance of 41 m
no. HEX15-1 to HEX15-6
Description
QUANTIFOIL with hexagonal holes
This type of QUANTIFOIL has been specially developed for the support of serial thin sections.
It is an optimum between mechanical stability on the one hand, and provides background-free area on the other.
The foil is thin enough to be able to interpret the parts of the sections which lie on the bar.
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Quantifoil Multi A
Quantifoil Multi A is a holey Film, in which a pattern of various hole sizes, shapes and arrangements is repeated.
no. S191-1 to S191-9
Description
Quantifoil Multi A
Is a holey film, in which a pattern of various hole sizes, shapes and arrangements is repeated.
In addition to circular holes, the pattern includes oval-shaped ones which appear round at high tilt angles (~70 ).
The diameters of the round holes are about 1, 1.4, and 2 m, and the bar widths range from 0.5 to 4 m.
The oval holes in the foil have a dimension of 8 x 2 m and 4 x 1 m.
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Quantifoil Mix Box
Content: 5x R1.2/1.3, 5x R2/1, 5x R3.5/1, Typ S7/2, 5x MultiA, 5x Carbon Film
no. S190-MIX
Description
Quantifoil – Mix Box
These Quantifoils in the this MixBox are only on copper grids.
Containing:
| 5x | Type R1.2/1.3 |
| 5x | Typ R2/1 |
| 5x | Type R3.5/1 |
| 5x | Type S7/2 |
| 5x | MultiA |
| 5x | Carbon Film |
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Quantifoil Array of 1 m Round Holes with Gold Islands R1/4
Quantifoil, Array of 1 m Diameter Holes with about 4 m Separation with Gold Islands
no. S200R14A300
Description
Quantifoil with gold Islands on Carbon Film
In case of a coating with holey carbon and Gold a closed gold layer can only have a minimum thickness of 4 – 5 nm because of technical limitations.
This is for transmission in the TEM, depending on the application, already too thick.
Therefore, Gold Islands will be made of about 2 nm thick, so that on the one hand molecules can bind with the gold and on the other hand obeservation between the islands is possible.
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Quantifoil Array of 2 m Round Holes with Gold Islands R2/2
Quantifoil, Array of 2 m Diameter Holes with about 4 m Separation with Gold Islands
no. S200R22A200 to N200
Description
Quantifoil with gold Islands on Carbon Film
In case of a coating with holey carbon and Gold a closed gold layer can only have a minimum thickness of 4 – 5 nm because of technical limitations.
This is for transmission in the TEM, depending on the application, already too thick.
Therefore, Gold Islands will be made of about 2 nm thick, so that on the one hand molecules can bind with the gold and on the other hand obeservation between the islands is possible.
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Description
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Quantifoil on Silicon Oxide 1 m Round Holes R1/4
Quantifoil, On Silicon Oxide, Array of 1 m Diameter Holes with 5 m Separation
no. S211R14A300 to N300
Description
QUANTIFOIL on Silicon Oxide.
This silicon oxide Film is made of amorphous SiO2 with a thickness of about 10 nm.
The layer is homogeneous, and robust.
Hole size: 1 m Hole spacing: 5 m
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